Charron DM & Zheng G
Nano Today, 2018
Nano-bio interactions are dynamic, interrelated, and overwhelmingly influence nanomedicine efficacy. Molecular imaging techniques such as fluorescence are essential for illuminating the nano-bio interface to guide nanomedicine development. With advances in reliable labelling of nanoparticles with fluorophores, integrated Förster resonance energy transfer (FRET) provides responsive fluorescence linked to nanomedicine integrity and function. In this review, we provide an overview of developments in the application of integrated FRET for imaging nanomedicines during nano-bio interactions. Furthermore, we discuss technical considerations and a general methodology for maximizing information from integrated FRET imaging and advancing its use as a routine tool in nanomedicine research.